论文部分内容阅读
目的评价石榴皮醇提物对小鼠肝脏的影响。方法将40只小鼠随机分成阴性对照组、石榴皮醇提物低、中、高剂量组(30,45,60 mg·kg-1)。所有小鼠在禁食12 h后,各剂量组给予相应剂量的石榴皮醇提物,阴性对照组给予等体积的蒸馏水。给药24 h后,所有小鼠麻醉后眼眶取血,麻醉脱臼处死。测定ALT,AST,LDH血清酶指标。留取肝组织测定MDA、GSH、GSSG评价氧化损伤的指标。留取1/2肝大叶经福尔马林溶液固定,HE染色观察小鼠肝脏病理改变。结果与阴性对照组比较,石榴皮醇提物中、高剂量组体质量、肝重、肝体比明显降低(P<0.05或P<0.01),ALT、LDH、MDA明显升高(P<0.05),GSH/GSSG明显降低(P<0.05),石榴皮醇提物高剂量组AST明显升高(P<0.05)。结论石榴皮醇提物能够引起小鼠肝脏的急性损伤,并且剂量越大,毒性越大。
Objective To evaluate the effect of ethanol extract of pomegranate peel on the liver of mice. Methods Forty mice were randomly divided into negative control group and low, medium and high dose groups of pomegranate alcohol extract (30, 45 and 60 mg · kg-1). All mice were fasted for 12 h, each dose group was given the corresponding dose of pomegranate alcohol extract, the negative control group was given an equal volume of distilled water. After administration for 24 h, all mice were anesthetized with orbital blood and anesthetized with dislocation. Determination of ALT, AST, LDH serum enzyme indicators. Liver samples were taken for determination of MDA, GSH and GSSG to evaluate oxidative damage. Half of the large lobe of liver was taken for formalin fixation and the pathological changes of the liver were observed by HE staining. Results Compared with the negative control group, the body weight, liver weight, liver and body weight were significantly decreased (P <0.05 or P <0.01), and the levels of ALT, LDH and MDA were significantly increased ), GSH / GSSG decreased significantly (P <0.05), and the content of AST in high-dose ethanol extract of pomegranate peel increased significantly (P <0.05). Conclusion Pomegranate peel alcohol extract can cause acute liver injury in mice, and the greater the dose, the greater the toxicity.