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目的:运用近红外光谱技术和化学计量学方法,建立巴戟天水溶性浸出物含量的近红外光谱测定方法。方法:冷浸法测定159批试验药材的水溶性浸出物含量,并采集近红外光谱数据,采用多元散射校正法、一阶导数法对光谱进行预处理,结合偏最小二乘法建立巴戟天中水溶性浸出物含量的近红外光谱分析模型,并对模型进行验证,得到水溶性浸出物含量的近红外光谱测定方法。结果:测定方法中建立的测定模型,内部交叉验证决定系数为0.9915,交叉验证校正标准偏差为0.4720,预测标准差为0.4890,交叉验证的标准偏差为0.8695。结论:建立的巴戟天水溶性浸出物近红外光谱测定方法稳定,准确可靠,可用于巴戟天药材的水溶性浸出物含量测定。
OBJECTIVE: To establish a method for the determination of water-soluble extracts of Morinda officinalis by near-infrared spectroscopy and chemometrics methods. Methods: The content of water-soluble extract in 159 batches of medicinal herbs was determined by cold soak method. Near-infrared spectroscopy data were collected. The spectra were pre-treated by multivariate scatter calibration method and first-order derivative method. Water-soluble extract content near-infrared spectral analysis model, and verify the model to obtain the content of water-soluble extract by near-infrared spectroscopy. Results: The determination model established in this method was 0.9915 for internal cross validation, 0.4720 for cross validation, 0.4890 for predictive standard deviation and 0.8695 for cross validation. Conclusion: The established method for determination of water-soluble extracts from Morinda officinalis is stable, accurate and reliable, and can be used for the determination of water-soluble extracts of Morinda officinalis.