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目的:研究药桑多糖对四氯化碳(CCl4)所致大鼠肝损伤的保护作用。方法:取健康Sprague-Dawley(SD)大鼠40只,根据体质量随机分为5组:对照组、CCl4损伤组、药桑多糖低(50 mg/(kg·d))、中(100 mg/(kg·d))、高(200 mg/(kg·d))剂量组,每组8只,连续灌胃7 d后,除对照组外,其余各组大鼠腹腔注射CCl4橄榄油溶液制造肝损伤模型。24 h后,取血清测定谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)活力以及胆红素含量,取各器官计算脏器指数并测定肝脏超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-Px)活力,以及丙二醛(malondialdehyde,MDA)、干扰素-γ(interferon-γ,IFN-γ)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-10(interleukin-10,IL-10)含量,并对肝脏进行组织切片观察。结果:100、200 mg/(kg·d)剂量的药桑多糖能显著增加大鼠体质量(P<0.05),降低肝脏指数(P<0.05)、肾脏指数(P<0.05)和脾脏指数(P<0.01),能显著抑制CCl4所致肝损伤大鼠血清ALT、AST活力和胆红素含量的升高(P<0.05);肝脏MDA、IFN-γ、TNF-α含量显著降低(P<0.05),SOD、CAT、GSH-Px活力以及IL-10含量显著升高(P<0.05)。50 mg/(kg·d)剂量的药桑多糖能显著降低肝损伤大鼠的肝脏指数、肾脏指数和脾脏指数(P<0.05),显著增加肝脏GSH-Px活力和IL-10含量(P<0.05),降低肝脏TNF-α含量(P<0.05)。结论:药桑多糖对CCl4诱导的大鼠肝损伤具有明显的保护作用,其肝脏保护作用与提高肝脏抗氧化能力及抑制肝脏炎症有关。
Objective: To study the protective effect of medicinal polysaccharide on liver injury induced by carbon tetrachloride (CCl4) in rats. Methods: Forty Sprague-Dawley (SD) rats were randomly divided into five groups according to the body weight: control group, CCl4 injury group, low dose of Chinese medicinal polysaccharide (50 mg / (kg · d) (kg · d) and high dose (200 mg / (kg · d)), each group had 8 rats in each group. After 7 days of continuous gavage, except for the control group, the rats in other groups were intraperitoneally injected with CCl4 olive oil solution To create a liver injury model. After 24 h, serum was taken to measure the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the content of bilirubin. The organs index was calculated and the superoxide dismutase , SOD, catalase (CAT), glutathione peroxidase (GSH-Px) activity, as well as malondialdehyde (MDA), interferon-γ , IFN-γ, TNF-α and IL-10 in the liver tissue were observed. (P <0.05), the index of kidney (P <0.05) and the index of spleen (P <0.05) were significantly lower than those of control P <0.01), and significantly inhibited the increase of ALT, AST and bilirubin levels in serum of CCl4-induced liver injury rats (P <0.05). The contents of MDA, IFN-γ and TNF- 0.05). The activities of SOD, CAT, GSH-Px and IL-10 were significantly increased (P <0.05). Phellinus igniarius at a dose of 50 mg / (kg · d) could significantly reduce the liver index, kidney index and spleen index (P <0.05), and significantly increase the hepatic GSH-Px activity and IL-10 content 0.05), reduce the content of liver TNF-α (P <0.05). CONCLUSION: Phellinus ignitus polysaccharides can protect CCl4-induced liver injury in rats, and its protective effect on the liver is related to improving liver anti-oxidant ability and inhibiting liver inflammation.