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目的 探讨急性心肌梗死 (AMI)早期经静脉途径移植骨髓基质干细胞对心功能的影响。方法 大鼠急性心肌梗死模型通过结扎冠状动脉前降支制作。同种异体大鼠骨髓基质干细胞体外培养、扩增、标记 ,并通过尾静脉于心肌梗死后 1d移植入AMI大鼠体内。分别进行心脏超声检查 ,以及组织学和免疫化学检测。结果 心肌梗死3周后 ,免疫组化分析表明部分干细胞迁移至梗死心肌周围并存活下来 ;干细胞移植组大鼠心功能较移植前及对照组明显改善 (P <0 0 5 )。结论 心肌梗死后 1d经静脉移植的骨髓基质干细胞可以归巢至梗死心肌处 ,并且可以改善损伤的心功能。
Objective To investigate the effects of transplanted bone marrow stromal cells (MSCs) via transvenous route on cardiac function in patients with acute myocardial infarction (AMI). Methods Acute myocardial infarction model was made by ligation of the anterior descending coronary artery. Allogeneic rat bone marrow stromal stem cells were cultured in vitro, expanded, labeled and transplanted into AMI rats via tail vein at 1 day after myocardial infarction. Echocardiography was performed separately, as well as histological and immunochemical tests. Results Three weeks after myocardial infarction, immunohistochemistry showed that some stem cells migrated to the peri-infarcted myocardium and survived. The cardiac function of the stem cell transplantation group was significantly improved compared with that before transplantation and the control group (P <0.05). Conclusion Intravenous transplantation of bone marrow stromal stem cells 1 h after myocardial infarction can be homing to infarcted myocardium, and can improve the cardiac function of injury.