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目的 探讨慢性疲劳综合征的神经免疫机制及运动的干预作用.方法 40只雄性Sprague-Dawley大鼠随机分为对照组,模型组、运动+模型组及运动组;采用睡眠剥夺加负重力竭性游泳方式建立慢性疲劳综合征模型;采用8周跑轮运动作为自动体力运动模型;用高效液相色谱法检测海马、枕叶皮层5-羟色胺(5-HT)含量,应用酶联免疫吸附(ELISA)法检测血清干扰素-γ(IFN-γ)和白细胞介素-4(IL-4)水平以及IFN-γ/IL-4比值变化.结果 模型组海马、枕叶皮层5-HT(11.28±5.13),(1.15±0.34)ng/g较对照组(33.56±6.42),(3.49±0.42)ng/g明显降低(P<0.01);血清IFN-γ(19.36±4.34)Pg/mL及IFN-γ/IL-4比值(0.96±0.62)较对照组明显下降(P<0.01);与模型组比较,运动+模型组5-HT(23.35±4.36),(2.67±0.51)ng/g明显增加;IFN-γ水平(37.58±5.13)pg/mL和IFN-γ/IL-4比值(2.65±0.91)明显增高(P<0.05);与对照组比较,运动组5-HT水平(54.27±5.31),(5.87±0.63)ng/g升高(P<0.05).结论 长期体力运动可作为一种有效的防护措施,可降低慢性疲劳对机体神经免疫功能的损害.“,”Objective To probe into the neuro-immune mechanism of chronic fatigue syndrome and the intervention effects of physical exercise. Methods Forty male SD rats were randomly divided into 4 groups: control group, model group, exercise model group and exercise group. The rat model of chronic fatigue syndrome was made by sleep deprivation and overload exhaustive swimming. Exercise model was made with 8-week spontaneous wheel running. The concentration of 5-hydroxytryptamine(5-HT) in hippocampus and occipital cortex was measured with high performance liquid chromatogra-phy. ELISA was employed to measure levels of interferon-γ(IFN-γ) and interleukine-4 (IL-4) in serum, and the ratio of IFN-γ/IL-4. Results Compared with the control group, the concentration of 5-HT, the level of IFN-γ and the ratio of IFN-γ/IL-4 were significantly decreased in model group (P < 0. 01). Compared with the model group, rats in exercise model group had higher levels of 5-HT, IFN-γ and a higher ratio of IFN-γ/IL-4 (P < 0. 05). The concertration of 5-HT was signifi-cantly increased in the exercise group than that of in the control group(P < 0. 05). Conclusion As an effective protection measure,long-term exercise can lower neuroimmunity injury caused by chronic fatigue syndrome.