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目的:探讨丙酮酸透膜与乳酸穿梭的关系。方法:不同运动能力的20名男性大学生分为运动组和对照组,每组10人,连续进行不同最大摄氧量百分比递增强度跑台运动,每级负荷运动3分钟,3分钟末取血,测试受试者血乳酸和丙酮酸浓度。结果:(1)运动组乳酸阈平均值出现在75%最大摄氧量强度,对照组出现在65%最大摄氧量强度,两组各级负荷血乳酸值与上一级负荷比较均有显著或非常显著性差异(P<0.05,P<0.01,P<0.001)。(2)对照组各级负荷下血丙酮酸浓度与上一级负荷比较均无显著性差异(P>0.05),运动组在75%、95%最大摄氧量强度下的血丙酮酸浓度与上一级负荷比较有显著性差异(P<0.05,P<0.01)。结果表明:(1)丙酮酸在乳酸阈之前不能或不易透过肌细胞膜。(2)提示丙酮酸透膜与运动强度、时相有关,其机制可能与乳酸穿梭到一定程度有关。
Objective: To investigate the relationship between pyruvate permeation and lactic acid delivery. Methods: Twenty male college students with different exercise capacity were divided into exercise group and control group, with 10 in each group. Continuous treadmill exercise with increasing percentage of maximum oxygen uptake intensity was carried out for 3 minutes for each exercise. Blood was taken at the end of 3 minutes, Subjects were tested for blood lactate and pyruvate concentrations. Results: (1) The mean lactic acid threshold of exercise group appeared at 75% of maximal oxygen uptake intensity while that of control group appeared at 65% of maximal oxygen uptake intensity. There was significant difference between the two groups Or very significant difference (P <0.05, P <0.01, P <0.001). (2) There was no significant difference of blood pyruvate concentration in the control group at all levels of loading (P> 0.05), the concentration of hemoglobin in exercise group at 75% and 95% There was a significant difference in upper level load (P <0.05, P <0.01). The results showed that: (1) pyruvate could not or hardly penetrate muscle cell membrane before lactate threshold. (2) Tip pyruvate membrane and exercise intensity, the phase, the mechanism may be related to the lactic acid shuttle to a certain extent.