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目的探讨改良羊水细胞染色体制片法在产前诊断中的效果。方法对孕14.3～37周具有产前诊断指征的孕妇,抽取羊水细胞培养6～7天,换液第2天取出观察,当有许多圆形透亮的细胞时,加入秋水仙素3h后收获。改良收获过程,将上清倒入离心管处理,瓶内细胞经低渗、预固定、固定后,刮下瓶壁细胞离心制片。结果692例羊水细胞培养成功691例,成功率99.86%;24～37孕周的成功率与<24孕周比较,差异无统计学意义(P>0.05);90.74%的收获时间是7～8天;有效分裂相数为20～118个以上的占97.83%,较原方法有显著提高(P<0.01)。结论该方法有效分裂相多、出结果快、成功率高。
Objective To investigate the effect of modified amniotic fluid cell chromosome preparation on prenatal diagnosis. Methods Pregnant women with prenatal diagnosis from 14.3 to 37 weeks of gestation were cultured for 6 to 7 days. After 2 days of fluid exchange, they were removed and observed. When there were many round translucent cells, colchicine was harvested after 3 hours . Improve the harvesting process, the supernatant was poured into centrifuge tube treatment, the cells within the bottle by hypotonic, pre-fixed, fixed, scraped bottle wall centrifuge tablets. Results The 692 cases of amniotic fluid cells were successfully cultured in 691 cases, with a success rate of 99.86%. The success rate of 24- to 37-week gestation was not significantly different from that of gestational age <24 (P> 0.05). The harvest time of 90.74% Day; the number of effective split phase is more than 20 ~ 118 accounted for 97.83%, compared with the original method was significantly increased (P <0.01). Conclusion The method is effective in dividing more phases, with faster results and higher success rate.