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采用QuEChERS/超高效液相色谱-串联质谱技术同时测定食品中13种植物生长调节剂残留。样品经乙腈提取,分散固相萃取净化,以乙腈和2 mmol/L甲酸铵溶液为流动相进行梯度洗脱,Waters C18色谱柱分离,采用电喷雾多反应监测模式,外标法定量。13种植物生长调节剂线性良好,线性相关系数均>0.99,该方法的检出限在0.1~1.5μg/kg之间,定量限在0.3~5.0μg/kg之间。添加10、20和50μg/kg 3个浓度水平,13种植物生长调节剂的平均回收率在75.9%~110.3%之间,相对标准偏差在0.1%~11.9%之间。将该技术应用于实际样品的测试,结果表明,方法简便、快速、灵敏度高,适用于食品中13种植物生长调节剂残留的同时测定。
Simultaneous determination of 13 plant growth regulators residues in food by QuEChERS / UHPLC tandem mass spectrometry. The sample was extracted with acetonitrile, purified by dispersive solid-phase extraction and eluted with acetonitrile and 2 mmol / L ammonium formate as mobile phase. The residue was separated on a Waters C18 column by electrospray multiple reaction monitoring and quantified by external standard method. Thirteen plant growth regulators had a good linearity with a linear correlation coefficient of> 0.99. The limits of detection of this method ranged from 0.1 to 1.5 μg / kg and the limits of quantification ranged from 0.3 to 5.0 μg / kg. The average recoveries of 13 plant growth regulators ranged from 75.9% to 110.3% with relative standard deviations between 0.1% and 11.9% at the concentrations of 10, 20 and 50 μg / kg. The technique was applied to the actual sample test, the results show that the method is simple, rapid, high sensitivity, suitable for simultaneous determination of 13 kinds of plant growth regulators residues in food.