论文部分内容阅读
目的探索增殖细胞核抗原(PCNA)与结直肠癌的组织学类型、临床分期及预后的关系。方法收集外科手术切除的96例结直肠癌石蜡标本。采用LSAB免疫组织化学染色法检测PCNA;按阳性细胞所占比例分为1～4级。1、2级为PCNA弱级,3、4级为PCNA强级。结果(1)PCNA在肠癌细胞中的表达明显增多,并与肿瘤的分化程度有关,分化差的肿瘤细胞PCNA表达增多;(2)PCNA表达与Dukes分期及淋巴结转移有关。DukesC期及有淋巴结转移者,PCNA表达明显增多;(3)PCNA表达与患者的性别、年龄、肿瘤部位、组织类型等因素无关;(4)PCNA表达与术后复发率及死亡率有关,PCNA强级者术后复发率及死亡率均显著高于弱级者。结论PCNA在常规的病理切片上能反映细胞的增殖活性,是判断结直肠癌患者预后及指导临床治疗的一个较为理想的参考指标。
Objective To explore the relationship between proliferating cell nuclear antigen (PCNA) and histological type, clinical stage and prognosis of colorectal cancer. Methods Surgical resection of 96 cases of colorectal cancer paraffin specimens were collected. PCNA was detected by LSAB immunohistochemical staining. The proportion of positive cells was 1 ~ 4. 1,2 for PCNA weak level, 3,4 for PCNA strong level. Results (1) The expression of PCNA in colorectal cancer cells was significantly increased, which was related to the degree of tumor differentiation. The expression of PCNA in poorly differentiated tumor cells was increased. (2) The expression of PCNA correlated with Dukes staging and lymph node metastasis. (3) The expression of PCNA was not related to the patient’s sex, age, tumor location and type of tissue; (4) The expression of PCNA correlated with postoperative recurrence rate and mortality, PCNA High-intensity postoperative recurrence rate and mortality were significantly higher than those with weaker. Conclusion PCNA can reflect the cell proliferative activity in routine pathological sections and is an ideal reference index for judging the prognosis of patients with colorectal cancer and guiding the clinical treatment.