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目的了解甘肃某市发热呼吸道综合征病例的细菌种类构成,建立省级呼吸道传染病监测技术平台。方法采用多重PCR法对甘肃省某市哨点医院2013年7月至2014年7月发热呼吸道综合征住院患者痰液样本进行肺炎链球菌、流感嗜血杆菌、军团菌、肺炎衣原体、肺炎支原体和百日咳杆菌检测,使用描述性统计学方法对阳性结果特征进行分析。结果检测100份痰液,任一病原阳性率为72.00%,肺炎链球菌(SP)检出率为62.00%、流感嗜血杆菌(HI)检出率为44.00%、肺炎衣原体(CT)检出率为2.00%、肺炎支原体(MP)检出率为6.00%,未检出军团菌(LP)和百日咳杆菌(BP),混合感染占42.00%。结论甘肃省某市发热呼吸道综合征住院病例细菌病原以SP和HI为主,两者混合感染多见。
Objective To understand the bacterial species composition of fever respiratory syndrome in a city of Gansu and establish a provincial monitoring platform for respiratory infectious disease. Methods Multiplex PCR was performed on sputum samples of hospitalized patients with pyrethroid respiratory syndrome from July 2013 to July 2014 in Sentinel Hospital of Gansu Province for Streptococcus pneumoniae, Haemophilus influenzae, Legionella pneumophila, Mycoplasma pneumoniae and Mycoplasma pneumoniae Bordetella pertussis test, descriptive statistics were used to characterize the positive results. Results The positive rate of any pathogen was 72.00% in 100 sputum samples. The detection rate of Streptococcus pneumoniae was 62.00% and that of Haemophilus influenzae was 44.00%. Chlamydia pneumoniae (CT) was detected The detection rate of Mycoplasma pneumoniae (MP) was 6.00% with no detection of Legionella (LP) and Bordetella pertussis (BP), and the mixed infection accounted for 42.00%. Conclusion The bacterial pathogens of fever respiratory syndrome in a city of Gansu Province are mainly SP and HI, and the mixed infection is common.