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目的观察慢性氟中毒对大鼠骨组织中转录因子Dlx5蛋白和mRNA表达水平的影响,探讨Dlx5在氟骨症发病机制中的可能作用。方法本研究用36只SD大鼠按性别随机分成3组,对照组(饮自来水,含氟<0.5mg/L)、低氟组(饮含氟5.0mg/L的自来水)、高氟组(饮含氟50mg/L的自来水),6个月后用氟离子选择电极法测定大鼠尿氟及骨氟含量,HE染色观察骨组织病理学改变,并采用原位杂交技术、免疫组织化学方法,对大鼠骨组织进行Dlx5蛋白及mRNA水平的定量分析。结果 HE染色显示染氟大鼠骨组织骨小梁增宽、骨皮质增厚等骨硬化表现;染氟大鼠骨组织Dlx5蛋白及mRNA表达水平显著高于对照组,差异有统计学意义(F低-蛋白=28.381,F高-蛋白=343.515,F低-mRNA=27.767,F高-mRNA=353.987,P均<0.05)。结论过量的氟可上调Dlx5蛋白及mRNA水平的表达,促进成骨细胞的增殖与分化,从而出现骨质硬化。
Objective To observe the effect of chronic fluorosis on the expression of Dlx5 protein and mRNA in rat bone and to explore the possible role of Dlx5 in the pathogenesis of skeletal fluorosis. Methods In this study, 36 SD rats were randomly divided into three groups according to their sex. The control group (tap water with fluoride <0.5 mg / L), low fluoride group (tap water containing 5.0 mg / L fluoride), high fluoride group Drinking water 50mg / L of tap water), 6 months after fluoride ion-selective electrode method for the determination of urinary fluoride and bone fluoride content, HE staining to observe the histopathological changes, and using in situ hybridization, immunohistochemical methods , Quantitative analysis of Dlx5 protein and mRNA levels in rat bone tissue. Results HE staining showed that trabecular bone was broadened and bone cortical thickening was observed in sclerosis rats. The expression of Dlx5 protein and mRNA in bone tissue of fluoridated rats was significantly higher than that of the control group (F low - Protein = 28.381, F high-protein = 343.515, F low -mRNA = 27.767, Fhigh-mRNA = 353.987, P <0.05). Conclusion Excessive fluoride can up-regulate the expression of Dlx5 protein and mRNA, promote the proliferation and differentiation of osteoblasts, and lead to osteosclerosis.