胚乳培养是植物组织培养中的一种。被子植物的胚乳是双受精的产物,具有双亲遗传物质的三倍染色体。通过胚乳培养可能获得三倍体的胚乳再生植株。我们选用小麦常规品种晋革早、莆麦3号及其杂种一代、二代的健康穗子,去颖壳,取出麦粒,在无菌的条件下依次放入70％的酒精中一分钟、0.1％的升汞中10分钟、5％的次氯酸钠中5分钟进行消毒,最后用无菌水冲洗3次。然后将胚切除,挤压出乳白色的胚乳,接种到诱导培养基上,置于26℃进行暗培养。10天后陆续长出愈伤组织,再转移到分化培养基上,光照400勒克斯,12/12小时光和暗循环(即每天12 Endosperm culture is a kind of plant tissue culture. The endosperm of the angiosperm is a product of double fertilization and has three chromosomes of the parental genetic material. It is possible to obtain triploid endosperm regenerated plants by endosperm culture. We choose the conventional varieties of wheat Jingezao early, Plum 3 and its hybrid generation, the second generation of healthy ears, to the husk, remove the grains, in order to aseptically placed in 70% alcohol for one minute, 0.1 % Mercuric chloride for 10 minutes, 5% sodium hypochlorite for 5 minutes for disinfection, and finally rinsed three times with sterile water. The embryos were then excised, extruded into milky white endosperm, inoculated into induction medium and placed in dark at 26 ° C. Ten days later, calluses were grown one after another, and transferred to a differentiation medium, and irradiated with 400 lux light for 12/12 hours light and dark cycle (ie, 12