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目的:探讨中药治疗对哮喘大鼠IL-4 mRNA和IFN-γ mRNA表达及IgE含量的影响。方法:24只Wistar大鼠,随机分为中药治疗组、空白对照组和哮喘模型组,利用卵白蛋白(OVA)及氢氧化铝制作大鼠哮喘模型,在雾化激发期,中药组用杏仁、甘草等中药制成的溶液雾化大鼠,其余组用生理盐水代替。采用免疫放射法、逆转录聚合酶链反应(RT-PCR)半定量法分别检测各组大鼠血清和肺泡灌洗液(BALF)中免疫球蛋白E(IgE)含量以及肺组织IL-4 mRNA和IFN-γ mRNA表达。结果:与空白对照组比较,模型组大鼠IgE含量和IL-4 mRNA的表达明显升高(P<0.05)。与模型组比较,中药治疗血清IgE的含量和IL-4 mRNA的表达明显降低(P<0.05),而IFN-γ mRNA表达则升高(P<0.05)。结论:IL-4可能参与哮喘的发生,而中药治疗可通过抑制IL-4 mRNA和上调IFN-γ mRNA的合成,改善哮喘炎症状态。
Objective: To investigate the effects of Chinese medicine treatment on the expression of IL-4 mRNA and IFN-γ mRNA and IgE in asthmatic rats. Methods: Twenty-four Wistar rats were randomly divided into traditional Chinese medicine group, control group and asthma model group. Ovarianbumin (OVA) and aluminum hydroxide were used to make rat asthma model. During the atomization period, the Chinese medicine group used almond, Rats made of licorice and other Chinese herbs atomized the rats, and the rest of the rats were replaced with physiological saline. Immunoglobulin and reverse transcription-polymerase chain reaction (RT-PCR) semi-quantitative methods were used to detect the levels of immunoglobulin E (IgE) and IL-4 mRNA in lung tissue in serum and bronchoalveolar lavage fluid (BALF) in each group. And IFN-γ mRNA expression. Results: Compared with the blank control group, the expression of IgE and IL-4 mRNA in the model group were significantly higher (P<0.05). Compared with the model group, serum IgE and IL-4 mRNA expression were significantly decreased (P<0.05), while IFN-γ mRNA expression was increased (P<0.05). Conclusion: IL-4 may be involved in the development of asthma. Traditional Chinese medicine can improve the inflammation of asthma by inhibiting IL-4 mRNA and up-regulating the synthesis of IFN-γ mRNA.