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用1.0g/100mL纤维素酶(OnozukaR-10),0.1g/100mL果胶酶(MecerzymeR-10)及10mmol/LCaCl2·2H2O,0.7mmol/LKH2PO4和0.5mol/L甘露醇的混合酶液,从14~18d苗龄的不结球白菜子叶上分离出高产率的原生质体。原生质体培养在KM8P1附加2,4-D0.5m/L、6-BA0.25mg/L、NAA0.5mg/L、葡萄糖9.0g/100mL、蔗糖1.0g/100mL、半乳糖0.03g/100mL液体培养基上,分裂旺盛。形成愈伤组织后