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目的 :比较人下颌骨和髂骨来源细胞的分子表达差异。方法 :从GEO公共数据库中,通过GEO2R软件分析获得在人下颌骨和髂骨来源细胞中差异表达的基因。应用DAVID和Gene MANIA在线数据库对这些基因之间的关系进行生物信息学分析。结果:从GEO数据获得了在人下颌骨和髂骨来源细胞中差异表达明显的293个基因,这些基因主要是同源盒基因,与骨骼发育密切相关。这些基因经DAVID数据库分析后,被富集到“skeletal system development”、“forebrain development”、“limb development”、“WNT信号通路”等不同的子集。经Gene MANIA在线数据库分析后,建立了MSX1、MSX2、HAND2、SIX1、PITX2、OSR2、PAX3和PRRX1等8个在人下颌骨与髂骨来源间充质细胞中表达差异明显的基因分子网络,这些基因通过共表达、共定位、遗传学相互作用等彼此关联。结论:下颌骨和髂骨来源间充质细胞存在差异化的分子调控,提示在应用髂骨细胞作为种子细胞对颌骨进行组织工程修复时,可考虑通过对髂骨间充质细胞进行基因修饰,以使其更接近颌骨间充质细胞特性。
OBJECTIVE: To compare the molecular differences in human mandible and iliac-derived cells. METHODS: Genes differentially expressed in human mandible and ilium-derived cells were obtained from the GEO public database by GEO2R software. Bioinformatic analysis of the relationships between these genes was performed using the DAVID and Gene MANIA online databases. RESULTS: 293 genes that were significantly differentially expressed in human mandible and ilium-derived cells were obtained from GEO data. These genes are mainly homeobox genes that are closely related to bone development. These genes were analyzed by DAVID database and enriched to different subsets such as “skeletal system development”, “forebrain development”, “limb development”, “WNT signaling pathway”. After GeneMania online database analysis, we established eight gene molecular networks with significant differences in expression of MSX1, MSX2, HAND2, SIX1, PITX2, OSR2, PAX3 and PRRX1 among human mandible and ilium-derived mesenchymal cells Genes are related to each other through co-expression, colocalization, genetic interactions, and the like. Conclusion: The molecular regulation of differentiated mesenchymal cells derived from the mandible and iliac bone suggests that the use of iliac cells as seed cells for tissue engineering repair of the jaw may be considered through genetically modified iliac mesenchymal cells , To make it more close to the characteristics of jaw mesenchymal cells.