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用高效液相色谱对化学方法合成的δ-睡眠诱导肽(DSIP)及其类似物(Tyr~1-DSIP,Phe~5-DSIP以及Tyr~1Phe~5-DSIP)的主要成份进行了分离,应用在线的紫外光谱和萤光光谱检测,并将分离出的色谱峰收集后进行氨基酸分析。
The main constituents of δ-sleep-inducing peptide (DSIP) and its analogs (Tyr ~ 1-DSIP, Phe ~ 5-DSIP and Tyr ~ 1Phe ~ 5-DSIP) synthesized by chemical method were separated by high performance liquid chromatography Application of online UV and fluorescence spectroscopy detection, and the chromatographic peaks were collected after amino acid analysis.