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目的探讨重组IFN-α蛋白联合endostatin基因对碱烧伤诱导角膜新生血管的抑制作用。方法兔眼球结膜下注射包有绿色荧光蛋白表达载体的脂质体,3 d后用激光共聚焦显微镜观察角膜绿色荧光蛋白表达。利用碱烧伤法制备兔眼角膜新生血管模型,球结膜下联合注射重组IFN-α蛋白及包有endostatin真核表达载体的脂质体,用裂隙灯显微镜观察对新生血管的抑制作用。结果实验组角膜有很强的绿色荧光,而在对照组则无荧光。联合应用重组IFN-α蛋白和endostatin基因治疗,第7、10、13天角膜新生血管长度、面积明显小于重组IFN-α蛋白和endostatin基因的单独治疗组(P<0.05)。结论重组IFN-α蛋白联合endostatin基因可有效抑制碱烧伤诱导角膜新生血管的生长。
Objective To investigate the inhibitory effect of recombinant IFN-α protein combined with endostatin gene on corneal neovascularization induced by alkali burn. Methods Rabbit eye subconjunctival injection of green fluorescent protein expression vector liposomes, 3 days after laser confocal microscopy of corneal green fluorescent protein expression. Rabbit corneal neovascularization model was prepared by alkaline burn method. The recombinant IFN-α protein and the liposome containing endostatin eukaryotic expression vector were injected subconjunctivally. The inhibitory effect on neovascularization was observed with slit lamp microscope. Results The experimental cornea had strong green fluorescence, but no fluorescence in the control group. Combination of recombinant IFN-α protein and endostatin gene therapy, the 7th, 10th and 13th day corneal neovascularization length and area were significantly less than the recombinant IFN-α protein and endostatin gene alone treatment group (P <0.05). Conclusion Recombinant IFN-α protein combined with endostatin gene can effectively inhibit the alkali burns induced corneal neovascularization.