论文部分内容阅读
本文介绍了一种用于血吸虫病间接荧光抗体试验的尾蚴玻片抗原的简易制备方法,证明经济而效果满意,具体步骤如下: 分别收集保种于纳塔尔多头乳鼠和光滑双脐螺或法氏双脐螺体内的埃及株或南非株曼氏血吸虫的混合尾蚴悬液,经0.5%若丹明(rhodamine)牛白蛋白(Difco)水溶液活体染色10分钟,用细铜丝网过滤,加入pH7.4PBS使成等渗,然后迅速边搅拌边滴加40%的福尔马林,使最终浓度为5%。所得尾蚴悬液以每15ml含3,000条尾蚴的批量分装,保存于4℃。制片时取5ml悬液(约含尾
In this paper, a simple preparation method of cercariae slide antigen for indirect fluorescent antibody test of schistosomiasis is introduced, which proves to be economical and satisfactory. The specific steps are as follows: collecting the seedlings of Natal polygamous suckling mice and smooth double umbilical snails A mixed cercariae suspension of the Egyptian strain or S. africanus Schistosoma japonicum in an A. fimbriatus strain was in vivo stained with 0.5% rhodamine bovine albumin (Difco) aqueous solution for 10 minutes, filtered through a thin copper mesh and added At pH 7.4 PBS, isotonicity was achieved and 40% formalin was rapidly added dropwise with stirring to a final concentration of 5%. The resulting cercariae suspension is dispensed in batches containing 15OmL of cercariae per 15 ml and stored at 4 [deg.] C. Preparation take 5ml suspension (about containing tail