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上海市肿瘤研究所对9例人原发性肝癌,1例癌旁组织,1例正常肝的Poly(A)~+RNA进行了分析。用各种基因探针作分子杂交,发现在6例原发性肝癌中,有二条增强表达的区带:2.2Kb和5.6Kb。肝癌组织较正常的mRNA在2.2Kb处有明显的增强。提示人N-ras基因的转录产物明显增强。癌旁,正常肝中N-ras基因的专一的mRNA很弱或低于检测水平。由于癌的发生是通过癌基因产物发生作用,因此N-ras基因在多数的人原发性肝癌中的表达明显增强,提示了N-ras基因是人原发性肝癌的重要转化基因之一。
Shanghai Institute of Cancer Research carried out analysis of 9 cases of human primary hepatocellular carcinoma, 1 para-cancer tissue, and 1 case of normal liver poly(A)~+RNA. Using a variety of gene probes for molecular hybridization, it was found that in 6 cases of primary liver cancer, there were two bands with enhanced expression: 2.2 Kb and 5.6 Kb. The mRNA of hepatocellular carcinoma was significantly increased at 2.2 Kb compared with normal mRNA. It suggested that the transcription product of N-ras gene was significantly enhanced. Beside the cancer, the specific mRNA of the N-ras gene in normal liver was weak or below the detection level. Because the occurrence of cancer is caused by oncogene products, the expression of N-ras gene is significantly enhanced in most human primary liver cancers, suggesting that N-ras gene is one of the important transforming genes of human primary liver cancer.