论文部分内容阅读
我们研制成4种对机体内重要生物活性物质(乙酰胆硷、组胺、5-羟色胺、胆酸)敏感的超微传感器-离子选择性微电极。它们对生物样品进行分析时具有快速、易行、设备简单的特点,而且响应迅速、选择性强、漂移小。电极尖端直径只有0.1μm左右,故可将2、3个不同的微型传感器组装在一起,并可将其插入活细胞内,在不严重干扰活细胞正常生理代谢的条件下,对活细胞内某些待测的离子活度做到定性、定量、定位及连续动态的记录和观察。这对机体内许多生物活性物质的定量分析极为重要,因为机体内这些生物活性物质与其相对应的水解酶经常处于一种快速的动态平衡之中,用普通单一点采样的分析方法很难精确地跟踪这一过程。离子选择性微电极能将待测细胞内某种离子的活度连续不断地转换成相应的电信号,经放大后直接输入计算机内进行分析和处理,从而实现分析过程中两个阶段一信息的转换、存贮和信息加工-处理的自动化。应用所研制的微电极研究活细胞及亚细胞水平离子的动态代谢,可在细胞水平深化对机体生理生化过程的了解,最终将为相应临床疾病的发生机理及药理学研究提供细胞水平的模型。应用乙酰胆硷选择性微电极对人体血红细胞内乙酰胆硷的活度进行了定量分析。结果显示,狂躁症患者血红细胞内乙酰胆硷/胆硷的活性约为正常值的4倍。应用对细胞内胆酸敏感的离子选择性电极,研究了胆酸和人体大肠癌的关系。结果表明,大肠癌细胞中胆酸活性约为正常大肠粘膜细胞中的4倍。
We developed four kinds of micro-sensors that are sensitive to important bioactive substances (acetylcholine, histamine, serotonin, cholic acid) in the body - ion selective microelectrode. They are fast, easy and simple to analyze for biological samples, and they respond quickly and with high selectivity and low drift. Electrode tip diameter of only about 0.1μm, so 2,3 different micro-sensors can be assembled together, and can be inserted into living cells, without serious interference with the normal physiological metabolism of living conditions of living cells within a Some of the ions to be measured to do qualitative, quantitative, positioning and continuous dynamic recording and observation. This is of crucial importance for the quantitative analysis of many bioactives in the body as these bioactives and their corresponding hydrolases in the body are often in a fast dynamic equilibrium and it is difficult to accurately Keep track of this process. Ion-selective microelectrodes can be measured cells within a certain kind of ion activity continuously converted to the corresponding electrical signals, amplified directly into the computer for analysis and processing, in order to achieve the analysis of two stages of a message Conversion, storage and information processing - automation of processing. Application of the developed microelectrode to study the dynamic metabolism of living cells and sub-cellular level ions can deepen the understanding of physiological and biochemical processes at the cellular level, and finally provide a model for the cellular level of the corresponding clinical disease pathogenesis and pharmacological research. Acetylcholine selective microelectrodes were used to quantitatively analyze the activity of acetylcholine in human red blood cells. The results showed that the activity of acetylcholine / choline in red blood cells of manic patients is about 4 times of normal. The application of ion-selective electrodes that are sensitive to intracellular bile acids has been used to study the relationship between bile acids and human colorectal cancer. The results showed that the activity of cholic acid in colorectal cancer cells was about 4 times higher than that in normal colorectal mucosa cells.