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以二甲基氨基偶氮苯（ＤＡＢ）诱发大白鼠肝癌为模型，观察了在诱癌过程中和肝癌形成后，肝细胞质膜上糖蛋白的变化。用不连续蔗糖密度梯度离心法制备肝细胞质膜，用ＣｏｎＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析法分离出肝细胞质膜糖蛋白，再进行ＳＤＳ－ＰＡＧＥ电泳分析。结果表明诱癌４ｗｋ，ｌ６ｗｋ的肝细胞质膜搪蛋白成份和正常肝比较无显著差异。肝癌细胞质膜上糖蛋白的ＳＤＳ－ＰＡＧＥ电泳图谱和正常肝比较，一些高分子量的糖蛋白缺失，而低分子量的糖蛋白含量增加．并显示了一条分子量为６．６１ＫＤ的新的糖蛋白带。
The model of liver cancer induced by dimethylaminoazobenzene (DAB) was used to observe the changes of glycoproteins on the plasma membrane of hepatocytes during the process of carcinogenesis and the formation of hepatocellular carcinoma. The plasma membrane of hepatocytes was prepared by discontinuous sucrose density gradient centrifugation. The plasma membrane glycoprotein was separated by ConA-Sepharose4B affinity chromatography and analyzed by SDS-PAGE. The results showed that there was no significant difference in the plasma membrane components of hepatocytes between 4wk and 16wk of cancer induction and normal liver. The SDS-PAGE of glycoproteins on the plasma membrane of liver cancer cells was compared with that of normal livers. Some high-molecular-weight glycoproteins were absent, while low-molecular-weight glycoproteins were increased. A new glycoprotein band with a molecular weight of 6.61KD was also shown.