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目的检测家族传播的口腔白色念珠菌基因多态性。方法采集35个家庭(119个样本)的口腔牙菌斑,采用PCR ITS1-ITS2基因分型方法,检测、分析家族传播的口腔白色念珠菌基因多态性。结果 18个家庭(18/35,61%),34个样本(34/119,28.6%)有白色念珠菌感染,11个家庭存在家族传播(11/18,61%)。在5个母子(父子)垂直传播的家庭成员中,白色念珠菌基因型均不一致。在3个呈水平传播的家庭成员中,两家基因型一致,1家不一致。在3个垂直-水平传播的家庭成员中,两家基因型一致,1家不一致。白色念珠菌家族传播基因型差异有显著统计学意义(χ2=26.571,P<0.01)。白色念珠菌感染与年龄、性别、学历、吸烟、饮酒、义齿和龋病均无显著相关。结论白色念珠菌在口腔定植,受宿主自身遗传背景影响较大,在家族垂直传播中呈明显的基因多态性。呈水平传播的白色念珠菌菌种具有较高的传染性,基因型可保持不变。
Objective To examine the family-transmitted Oral Candida albicans gene polymorphism. Methods Tooth plaque was collected from 35 families (119 samples). PCR ITS1-ITS2 genotyping method was used to detect and analyze the family-transmitted Oral Candida albicans gene polymorphism. Results 18 families (18/35, 61%), 34 samples (34 / 119,28.6%) had Candida albicans infection, and 11 families had family transmission (11 / 18,61%). Candidate Candida genotypes were inconsistent among 5 family members (father and son) that spread vertically. Of the three horizontally-spread family members, the two genotypes were identical and one was inconsistent. Of the three vertically-level members of the family, the two genotypes were identical and one was inconsistent. Candida albicans family transmission genotype differences were statistically significant (χ2 = 26.571, P <0.01). Candida albicans infection and age, gender, education, smoking, alcohol, denture and caries were not significantly correlated. Conclusion Candida albicans colonization in the mouth, the host of their own genetic background greater impact on the family vertical transmission was significant genetic polymorphism. Candida albicans species that spread horizontally are highly contagious and their genotypes can remain unchanged.