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为研究京大戟醋制前后不同极性部位对斑马鱼胚胎肝、胃肠毒性的影响,实验以95％乙醇提取生、醋京大戟,经石油醚、二氯甲烷、乙酸乙酯依次萃取得不同极性部位.将受精后12 h(12 hpf)的斑马鱼胚胎暴露于不同浓度的溶液中持续至受精96 h(96 hpf),对斑马鱼胚胎的肝、胃肠进行毒性观察.结果显示,毒性随着样品浓度的增加而增强.京大戟醋制前后各极性部位对斑马鱼胚胎的肝、胃肠毒性主要表现为肝脏发育缓慢、肝面积变小、肝脏变性发黑、卵黄囊水肿、吸收延迟、出血、胃肠道蠕动减缓、胃肠道杯状细胞分泌功能异常等,醋制后毒性反应降低.其中石油醚和二氯甲烷部位肝、胃肠毒性作用较为明显,乙酸乙酯部位毒性较弱.以上结果说明,京大戟醋制后毒性降低,且石油醚、二氯甲烷部位是其产生肝、胃肠毒性的主要部位.“,”To study the effects of different fraction of Euphorbiae Pekinensis Radix before and after processing with vinegar on liver and gastrointestinal toxicity of zebrafish embryos, the zebrafish embryos after fertilized 12 h (12 hpf) were exposed to different concentrations of solution until 96 h (96 hpf), for observation of the toxicity response of the liver and gastrointestinal of individual zebrafish embryos. The results showed that toxicity increased in a dose-dependent manner. The liver and gastrointestinal toxicity of the zebrafish embryos in various polar fractions of Euphorbiae Pekinensis Radix before and after processing with vinegar was mainly manifested as slow liver development, smaller liver area, edema of yolk sac, delayed absorption, slowing of gastrointestinal motility, abnormal function of gastrointestinal goblet cell secretion. In addition, the toxicity of different polarity was followed by petroleum ether, dichloromethane, ethyl acetate. The above results indicated that the toxicity was reduced after processing with vinegar, and the fractions of petroleum ether and methylene chloride were the main sites responsible for liver and gastrointestinal toxicity.