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我们已有的工作揭示 ,在混合皮肤移植的体外模型MELC中 ,自体角朊细胞可通过间接递呈同种异体抗原诱导免疫抑制。本文在此基础上 ,进一步分析其中Th1和Th2相关细胞因子含量的变化。结果发现 :(1)向MELC引入自体角朊细胞后约 32h ,原同种异体应答中出现的IFN γ和IL 2分泌格局开始让位于IL 4和IL 10 ,6 4h后IFN γ和IL 2完全消失 ,表明发生了淋巴细胞Th1向Th2亚群的极化 ;(2 )单抗封闭试验表明 ,IL 10在其中起关键作用 ;(3)经自体和异体角朊细胞调变过的淋巴细胞对MELR的作用相反 ,前者抑制 ,后者促进。这是首次从T亚群相互作用上探索并揭示混合皮肤移植引起局部免疫耐受的机制。
Our work has revealed that autologous keratinocytes can induce immunosuppression by indirect presentation of allogenic antigens in a mixed skin graft in vitro model MELC. Based on this, we further analyze the changes of Th1 and Th2 related cytokines. The results showed that: (1) about 32h after the introduction of autologous keratinocytes into MELC, the secretion pattern of IFNγ and IL-2 in the original allogeneic response began to be shifted to IL-4 and IL-10; IFNγ and IL-2 Completely disappeared, indicating the occurrence of polarization of the Th1 to Th2 subsets of lymphocytes; (2) mAb blocking assays have shown that IL 10 plays a key role therein; (3) lymphocytes modified by both autologous and allogeneic keratinocytes Contrary to MELR, the former inhibits the latter and the latter promotes it. This is the first time that T-subpopulation interaction has been explored to reveal the mechanism by which mixed skin grafts cause local immune tolerance.