目的　测定在缺氧预适应中脑组织 PKCα、β1 、ε、ζ表达的变化 ,探讨缺氧预适应形成机制。方法　采用流式细胞仪多克隆抗体间接免疫荧光技术测定。结果　随着缺氧次数的增加 ,PKCα、β1 、ε、ζ表达各亚型程度不同地持续增强 ,但 4次缺氧组有所回降。结论　 PKC可能通过减弱其所引发的一系列病理变化 ,参与缺氧预适应 Objective To determine the expression of PKCα, β1, ε, ζ in hypoxic preconditioning brain and explore the mechanism of hypoxic preconditioning. Methods Flow Cytometry Polyclonal antibody indirect immunofluorescence assay. Results With the increase of hypoxia, the expression of PKCα, β1, ε, ζ increased continuously with different subtypes. Conclusions PKC may be involved in hypoxic preconditioning by attenuating the series of pathological changes