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目的:了解人博卡病毒(human bocavirus,HBoV)在南京地区呼吸道感染患儿中的致病情况。方法:选取2009年11月～2010年6月因呼吸道感染住院的患儿鼻咽吸取物标本共100份,采用聚合酶链反应(PCR)方法检测HBoV的NP1基因片段,并对阳性产物进行测序,利用多功能分析软件DNAstar进行同源性分析,MEGA软件绘制遗传进化树。分析感染患儿的临床资料。结果:在100例鼻咽吸取物标本中共检测到5例阳性,阳性率为5%。DNAStar分析发现5例阳性标本之间核苷酸同源性达99.7%～100.0%,与其他国家HBoV的株同源性为99.4%～100.0%。临床资料显示这5例患儿临床症状主要表现为咳嗽、喘息、发热等,临床诊断为肺炎2例、毛细支气管炎1例、支气管炎1例及喘息性支气管炎1例。患儿经常规治疗后均痊愈出院。结论:南京地区呼吸道感染患儿中有HBoV的流行。
Objective: To understand the pathogenicity of human bocavirus (HBoV) in children with respiratory tract infection in Nanjing. Methods: A total of 100 specimens of nasopharyngeal aspirates from hospitalized children with respiratory tract infection from November 2009 to June 2010 were selected. The NP1 gene fragment of HBoV was detected by polymerase chain reaction (PCR) and the positive products were sequenced , Homology analysis was performed using DNAstar, a multifunctional analysis software, and MEGA software was used to map the phylogenetic tree. Analysis of children with clinical data. Results: In 100 samples of nasopharyngeal aspirates, 5 cases were detected positive, the positive rate was 5%. DNAStar analysis showed that the nucleotide homology among 5 positive samples was 99.7% ~ 100.0%, and the homology with other countries was 99.4% ~ 100.0%. Clinical data show that the clinical manifestations of these 5 patients mainly manifested as cough, wheezing, fever, clinical diagnosis of pneumonia in 2 cases, bronchiolitis in 1 case, bronchitis in 1 case and asthmatic bronchitis in 1 case. Children after regular treatment were cured. Conclusion: The prevalence of HBoV in children with respiratory tract infection in Nanjing area.