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目的 :探讨阿司匹林 (ASA)对胃癌细胞株SGC 790 1作用的相关机理 ,为寻找其新的药物用途奠定理论基础。方法 :用MTT法检测药物对细胞的抑制率 ;用流式细胞仪测定细胞周期 ;用琼脂糖凝胶电泳法检测细胞凋亡 ;3H TdR同位素示踪测定细胞DNA合成。结果 :ASA对SGC 790 1细胞株的细胞作用具有量效和时效依赖关系。ASA对细胞DNA合成有抑制作用 ,并可使SGC 790 1细胞周期中S期及G2 /M期比例升高 ,G1期比例下降 ,呈一定的剂量效应关系。琼脂糖凝胶电泳结果未见典型的阶梯状凋亡条带。结论 :阿司匹林对SGC 790 1细胞株存在着细胞毒作用 ,其细胞毒作用可能与抑制DNA的合成、阻止细胞周期有关系
Objective: To explore the mechanism of aspirin (ASA) on gastric cancer cell line SGC 790 1, and to lay a theoretical foundation for finding new drug use. Methods: MTT assay was used to detect the inhibitory rate of the cells; flow cytometry was used to measure the cell cycle; apoptotic cells were detected by agarose gel electrophoresis; 3H TdR isotope tracer was used to determine the cell DNA synthesis. RESULTS: The cellular effects of ASA on SGC7901 cells were dose- and time-dependent. ASA inhibited DNA synthesis and increased the ratio of S phase and G2/M phase in SGC7901 cell cycle, and decreased the proportion of G1 phase, showing a dose-response relationship. Agarose gel electrophoresis showed no typical stepped apoptotic bands. Conclusion : Aspirin has cytotoxicity on SGC7901 cell line. Its cytotoxicity may be related to the inhibition of DNA synthesis and cell cycle arrest.