论文部分内容阅读
目的:建立同时测定人血清中苯妥英、卡马西平及其代谢物10,11-环氧化卡马西平浓度的HPLC法。方法:用含内标氯唑沙宗的乙腈沉淀样品中蛋白,取上清液进样。分析柱为YWG-C_(18)(4.6mm×200mm,10μm),流动相为乙腈-甲醇-水(13:25:62),检测波长为214 nm。结果:卡马西平、10,11-环氧化卡马西平和苯妥英的标准曲线范围分别为0.4～16,0.2～8,1～40μg·mL~(-1),最低检测浓度分别为0.2,0.1,0.5μg·mL~(-1)。三者的方法回收率近100％,日内和日间精密度均小于6％,内源性物质和常用药物均不干扰测定。结论:该方法操作简便,灵敏、准确,适用于治疗药物监测。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of phenytoin, carbamazepine and its metabolites 10,11-epoxidized carbamazepine in human serum. Methods: Acetonitrile containing internal standard chlorzoxazone was used to precipitate the protein in the sample, and the supernatant was injected. The analytical column was YWG-C 18 (4.6 mm × 200 mm, 10 μm). The mobile phase consisted of acetonitrile-methanol-water (13:25:62) and the detection wavelength was 214 nm. Results: The calibration curves of carbamazepine, 10,11-carbamazepine and phenytoin were 0.4 ~ 16, 0.2 ~ 8 and 1 ~ 40μg · mL -1, respectively. The lowest detection concentrations were 0.2, 0.1, 0.5μg · mL -1. The recovery rate of the three methods is nearly 100%, the intra-day and inter-day precision are less than 6%, endogenous substances and commonly used drugs do not interfere with the determination. Conclusion: The method is simple, sensitive, accurate and suitable for the treatment of drug monitoring.