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In this work,a metal-organic frameworks material MIL-88 was prepared easily using solvent-thermal method,and was first found to have catalytic activities similar to those of biological enzymes such as catalase and peroxidase.The material was characterized by XRD,SEM,TEM,EDX,FT-IR techniques and an N_2 adsorption method.It exhibited peroxidase-like activity through catalytic oxidation of the peroxidase substrate 3,3’,5,5’-tetramethylbenzidine(TMB) in the presence of H_2O_2,producing a blue-colored solution.Under optimal conditions,the absorbance at 652 nm is linearly correlated with the concentration of H_2O_2 from 2.0×10~(-6) mol/L to 2.03×10~(-5) mol/L(R~2=0.981) with a detection limit of 5.62×10~(-7) mol/L(S/N=3).More importantly,a sensitive and selective method for ascorbic acid detection was developed using this material as a catalyst.The analytical method for ascorbic acid detection was observed to have a linear range from 2.57×10~(-6) mol/L to 1.01×10~(-5) mol/L(R~2=0.989) with a detection limit of 1.03×10~(-6) mol/L(S/N=3).This work suggests MOFs have advantages of preparing biomimetic catalysts and extends applications of the functional MOFs in the field of biosensor.
In this work, a metal-organic frameworks material MIL-88 was prepared using using solvent-thermal method, and was first found to have catalytic activities similar to those of biological enzymes such as catalase and peroxidase. The material was characterized by XRD, SEM , TEM, EDX, FT-IR techniques and an N 2 adsorption method. It exhibited peroxidase-like activity through catalytic oxidation of the peroxidase substrate 3,3 ’, 5,5’-tetramethylbenzidine (TMB) in the presence of H 2 O 2, producing a blue-colored solution.Under optimal conditions, the absorbance at 652 nm is linearly correlated with the concentration of H 2 O 2 from 2.0 × 10 -6 mol / L to 2.03 × 10 -5 mol / L (R 2 = 0.981) with a detection limit of 5.62 × 10 -7 mol / L (S / N = 3) .More importantly, a sensitive and selective method for ascorbic acid detection was developed using this material as a catalyst.The analytical method for ascorbic acid was observed to have a linear range from 2.57 × 10 -6 mol / L to 1.01 × 10 -5 mol / L (R 2 = 0.989) This work suggests MOFs have advantages of preparing biomimetic catalysts and extends applications of the functional MOFs in the field of biosensor.