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报道以荧光薄层扫描法(TLCS)测定补益中药中磷脂酰胆碱(PC)的含量。以丙酮和氯仿—甲醇—冰醋酸—乙醇—水(25:4:6:2:0.5),2次同向展开,在碱性硅胶H板上分离PC,经DPH显色后,进行TLCS定量,回收率97.2%,RSD=1.89%,方法简便,灵敏,快速。国内外来见报道。
It was reported that the content of phosphatidylcholine (PC) in traditional Chinese medicine was determined by fluorescent thin layer scanning (TLCS). Acetone and chloroform-methanol-glacial acetic acid-alcohol-water (25:4:6:2:0.5) were developed twice in the same direction. PC was separated on basic silica gel H plates and developed by DPH. TLCS quantification, the recovery rate of 97.2%, RSD = 1.89%, the method is simple, sensitive and fast. Domestic and foreign reports.