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选用大叶密合和5个对青枯病具有不同抗性水平的烟草品种,按完全双列杂交设计,采用Griffi ng方法 I及Hayman方法进行遗传分析,并应用植物数量性状主基因+多基因混合遗传模型对大叶密合(抗病品种)×长脖黄(感病品种)组合P1、P2、F1和F2等4个世代群体的青枯病抗性进行了联合分析。结果表明:参试品种的青枯病抗性为细胞核遗传;大叶密合×长脖黄组合的青枯病抗性遗传符合2对加性-显性-上位性主基因+加性-显性多基因遗传模型(E-1),主基因的加性和显性效应值分别为0.5013、-0.3023和1.6439、0.8401,多基因的加性和显性效应值分别为-1.3989和-1.7798,主基因遗传率63.95%,利用大叶密合进行抗病育种,适宜在分离晚世代进行选择并加大后代筛选群体。大叶密合与NC95的抗性遗传存在差异,后者的抗性表现为加性遗传。
The tobacco varieties with large leaf density and five varieties with different resistance to bacterial wilt were selected and analyzed by complete double crosses. The genetic analysis was made by Griffi ng method I and Hayman method, and the major gene + polygene The mixed genetic model was used to analyze the bacterial wilt resistance of four generations of P1, P2, F1 and F2 combinations of the large-scale closely related (resistant variety) × long-necked yellow (susceptible variety) combinations. The results showed that the resistance to bacterial wilt of the tested cultivars was nuclear inheritance. The inheritance of resistance to bacterial wilt disease was consistent with 2 pairs of additive-dominance-epistatic major genes plus additive-significant The additive and dominant effects of the major genes were 0.5013, -0.3023 and 1.6439, 0.8401, respectively. The additive and dominant effects of the multigene were -1.3989 and -1.7798, respectively. The heritability of the major gene is 63.95%. Breeding with resistance to disease by big leaf close fit is suitable for selecting the later generations and increasing the population of the next generation. There is a difference in the genetic inheritance of resistance between NCL and NCL. The resistance of the latter is additively inherited.