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本研究旨在分析中国人群急性髓系白血病(acute myeloid leukemia,AML)患者死亡相关蛋白激酶(deathassociated protein kinase,DAPK)基因启动子的甲基化状况及其临床相关性。应用甲基化特异性PCR(methylation-specific PCR,MSP)技术对112例AML患者骨髓标本dapk基因甲基化状态进行检测。结果表明:13例对照组均未发生dapk基因启动子甲基化,而112例AML患者中82例(73.2%)发生dapk基因甲基化,dapk基因甲基化改变与患者的性别、年龄、白细胞、血红蛋白、血小板计数、AML分型以及染色体异常等结果均无相关性(p>0.05)。结论:dapk基因甲基化是AML中的一个常见分子事件。
This study aimed to analyze the methylation status and its clinical significance of the promoter of death-associated protein kinase (DAPK) in Chinese patients with acute myeloid leukemia (AML). Methylation-specific PCR (MSP) was used to detect the methylation status of dapk gene in 112 patients with AML. The results showed that: dapk gene promoter methylation was not found in all 13 control cases, while dapk gene methylation was found in 82 (73.2%) of 112 AML patients. The dapk gene methylation was associated with gender, age, No correlation was found between leucocyte, hemoglobin, platelet count, AML typing and chromosomal abnormalities (p> 0.05). Conclusion: dapk gene methylation is a common molecular event in AML.