日常病理工作中,会经常遇到含骨及钙化组织制作石蜡切片的病例。但是骨及钙化组织非常坚硬,进行石蜡制片的整个过程程序繁多,其中以组织的固定、取材、脱钙、除酸、切片、染色等环节最为重要。当中任何一个环节出现问题,均可影响切片的质量,而直接影响到病理报告的准确性 In routine pathology, cases of paraffin-embedded bone and calcified tissue are often encountered. However, the bone and calcified tissue is very hard, the whole process of paraffin preparation procedures are numerous, including the organization of fixed, drawing, decalcification, acid removal, slicing, staining and other aspects of the most important. Any one part of the problems, can affect the quality of the slice, which directly affects the accuracy of the pathology report