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目的 :研究肿瘤坏死因子α(TNF α)和干扰素α(IFN α)对耐阿霉素 (ADR)K5 6 2细胞株耐药性的逆转作用。方法 :应用MTT法 ,分析TNF α和IFN α(均为 10 0U/ml)分别处理K5 6 2 /A0 2前后 ,K5 6 2 /A0 2对ADR敏感性的变化。采用半定量RT PCR和免疫组织化学染色法 ,观察mdr1基因的表达 ;应用流式细胞技术检测细胞内ADR浓度的变化。结果 :TNF α与IFN α分别处理K5 6 2 /A0 2细胞 ,2 4h后逆转倍数为 6和 5 ,处理 48h后 ,逆转倍数分别为 10倍和 8倍 ,均具有显著差别 ;但 72h后 ,逆转倍数反而减小。在TNF α和IFN α孵育后 2h ,K5 6 2 /A0 2细胞内ADR的积累分别增加 2 .2和 1.8倍 ,而对K5 6 2 /S无明显增加ADR积累作用。结论 :TNF α和IFN α(10 0U/ml)对K5 6 2 /A0 2的耐药性具有明显的逆转活性 ,其作用似乎呈时间依赖性。由于低剂量TNF α和IFN α毒性微小 ,因此 ,具有一定的实际临床应用意义。
Objective: To investigate the reversal effect of tumor necrosis factor α (TNF α) and interferon α (IFN α) on drug resistance of adriamycin resistant K5 6 2 cell line. Methods: MTT assay was used to analyze the changes of ADR sensitivity of K562 / A02 before and after K562 / A02 treatment with TNFa and IFNa (both 10U / ml). Semi-quantitative RT-PCR and immunohistochemical staining were used to observe the expression of mdr1 gene. Flow cytometry was used to detect the intracellular ADR concentration. Results: After treatment with TNFα and IFNα for K562 / A0 2 cells, the reversal multiples were 6 and 5 after 24 hours, respectively. After 48 hours of treatment, the reversal multiples were 10 times and 8 times, respectively. Instead, the fold decreases. The accumulation of ADR in K562 / A02 cells increased by 2.2 and 1.8 times, respectively, 2 h after TNFα and IFNα incubation, but no significant increase in ADR accumulation on K5 6 2 / S cells. CONCLUSION: TNFα and IFNα (10 0U / ml) have obvious reversal activity on K562 / A02 resistance, which seems to be time-dependent. Due to the low toxicity of TNFα and IFNα, it has some practical clinical significance.