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背景与目的:胎盘部位滋养细胞肿瘤(placental site trophoblastic tumor,PSTT)是一种特殊类型的妊娠滋养细胞肿瘤。该研究旨在建立人PSTT细胞系,鉴定其基本特性。方法:通过手术切除的PSTT组织标本原代培养,纯化并传代,建立人PSTT细胞系。鉴定方法包括:观察细胞形态,CCK-8法绘制细胞生长曲线,进行染色体核型分析,细胞与肿瘤组织短串重复序列(short tandem repeat,STR)分析,以及免疫组织化学染色。结果:建立稳定传代的人PSTT细胞系PSTT-1。PSTT-1细胞单层贴壁生长,失去接触抑制性,呈多突起的纺锤形结构。体外培养生长良好,每5 d传代1次,目前已传至30余代。染色体核型分析结果为正常女性核型:46,XX。PSTT-1与肿瘤组织STR分析结果高度匹配。PSTT-1细胞表达β-catenin、CD146、人绒毛膜促性腺激素(human chorionic gonadotrophin,hCG)、人胎盘生乳素(human placental lactogen,hPL)和Muc4。结论:该研究成功建立人PSTT细胞系PSTT-1,为PSTT的研究提供了体外实验模型。
BACKGROUND & OBJECTIVE: Placental site trophoblastic tumor (PSTT) is a special type of gestational trophoblastic tumor. The aim of the study was to establish a human PSTT cell line to identify its essential characteristics. Methods: PSTT tissue samples were primary cultured, purified and passaged to establish human PSTT cell line. Identification methods include: observation of cell morphology, cell growth curve by CCK-8 method, chromosome karyotype analysis, short tandem repeat (STR) analysis of cells and tumor tissues, and immunohistochemical staining. RESULTS: Stable passaged human PSTT cell line PSTT-1 was established. PSTT-1 cells monolayer adherent growth, loss of contact inhibition, was multi-protruding spindle-shaped structure. Well cultured in vitro, every 5 d passage 1, has now spread to more than 30 generations. Chromosome karyotype analysis results for normal female karyotype: 46, XX. PSTT-1 and STR analysis of tumor tissue match the results. PSTT-1 cells express β-catenin, CD146, human chorionic gonadotrophin (hCG), human placental lactogen (hPL) and Muc4. Conclusion: This study successfully established human PSTT cell line PSTT-1, which provided an in vitro experimental model for the study of PSTT.