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Objective To investigate the mutant frequency and polymorphism of HIV-1 resistance CCR5-△32, CCR2-64I, SDF1-3A alleles in Jingpo and Dai nationalities of Yunnan Methods The study population included 101 Dai and 113 Jingpo ethnical subjects.The genotypes were respectively detected by polymerase chain reaction (PCR) or by PCR/RFLP (restriction fragment length polymorphism) assay. Mutant frequencies were calculated and allelic polymorphism of the three genes in population was analyzed by U test.Results We didnt find CCR5-△32 mutant in either Dai or Jingpo nationality. In Dai minority, the allele frequency of CCR2-641 was 21.00% and that of SDF1-3A was 20.30%. In Jingpo minority, the allele frequency of CCR2-64I was 16.37% and that of SDF1-3 A was 17. 70%.Conclusion Dai and Jingpo nationality of Yunnan might have a high genetic susceptibility to HIV-1 (including R5 and X4 HIV strains) since we didnt find CCR5- △32 and the frequency of SDF1-3A was much lower than that of Han nationality as was reported in other research papers.