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用大肠杆菌ＤＨ５α菌体蛋白免疫家兔制备抗血清，建立了双抗体夹心ＥＬＩＳＡ方法。经初步测定，该方法的灵敏度为１．５ｎｇ／ｍｌ，与间接ＥＬＩＳＡ法的灵敏度相似，比聚丙烯酰胺凝胶电泳的考马斯亮兰染色方法的灵敏度高将近７００倍，比银染色方法的灵敏度高将近３０倍。在２０～１００００ｎｇ／ｍｌ范围内呈直线关系，直线相关系数为０．９９６。经八次重复测定，显示很好的重复性。该方法可用于测定基因工程产品中ＤＨ５α菌体蛋白的残余量。
Antiserum was prepared by immunizing rabbits with E. coli DH5α bacterial protein and a sandwich ELISA method was established. After preliminary determination, the sensitivity of this method is 1.5 ng / ml, which is similar to the sensitivity of indirect ELISA, and is nearly 700 times higher than the Coomassie brilliant blue staining method of polyacrylamide gel electrophoresis, which is more sensitive than the silver staining method Nearly 30 times. In the range of 20 ~ 10000ng / ml showed a linear relationship, the linear correlation coefficient of 0.996. After eight replicates, showed good repeatability. This method can be used to determine the residual amount of DH5 [alpha] bacterial protein in genetically engineered products.