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目的:研究健脾补肾方对原位移植瘤小鼠生存状况的影响、瘤体的抑制作用及对肿瘤组织中Ki67蛋白表达的影响。方法:24只BALB/c小鼠盲肠原位癌造模,造模后随机分组,分为健脾补肾方高、中、低剂量(43.700、21.850、10.925g/kg)组和对照组,每组6只,灌胃给药,每天1次,共14次,连续给药2周。观察各组小鼠的生长状况、给药结束后比较瘤体重量;免疫组化和Western blot检测增殖指标Ki67。结果:与对照组比较,健脾补肾方高、中剂量组瘤重降低(P<0.05);免疫组化及Western blot检测均表明健脾补肾方可以下调Ki67(P<0.05)。结论:健脾补肾方高、中剂量组可以改善小鼠生存状况、抑制肿瘤细胞增殖、下调Ki67基因蛋白的表达。
Objective: To study the effect of Jianpi Bushen Recipe on the survival of transplanted tumor in situ, the inhibition of tumor and the effect on the expression of Ki67 protein in tumor tissue. Methods: Twenty-four BALB / c mice were made into cecal carcinoma in situ and were randomly divided into three groups: high, medium and low dose (43.700,21.850,10.925g / kg) Group 6, intragastric administration, once daily, a total of 14 times, continuous administration for 2 weeks. The growth of mice in each group was observed. The weight of tumor was compared after the administration. The proliferation index Ki67 was detected by immunohistochemistry and Western blot. Results: Compared with the control group, the tumor weight of Jianpishenzheng prescription group was lower than that of the control group (P <0.05). The results of immunohistochemistry and Western blot showed that Jianpi Bushen recipe could down-regulate Ki67 (P <0.05). Conclusion: Jianpi Bushen Fang high and medium dose groups can improve the survival of mice, inhibit the proliferation of tumor cells and down-regulate the expression of Ki67 gene.