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报道了利用Al3+作为柱前衍生试剂,高效液相色谱分离荧光检测法检测测定四环素类药物的新方法。探讨了四环素类药物-Al3+络合物的衍生条件及其色谱分离条件对荧光强度的影响,3种药物的检出限达70-150pg。
Reported the use of Al3 + as a pre-column derivatization reagent, high performance liquid chromatography separation of fluorescence detection method for the determination of tetracyclines new method. The effects of the derivatization conditions of tetracycline-Al3 + complexes and their chromatographic conditions on the fluorescence intensity were investigated. The detection limits of the three drugs ranged from 70 to 150 pg.