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目的利用多种生物信息学方法,研究SH3PXD2B基因序列信息和蛋白结构,为今后进一步验证该基因在口腔颌面头颈部的功能提供依据。方法从Genbank得到SH3PXD2B序列,采用基因结构分析,预测该蛋白的亚细胞定位、二级结构、三级结构、功能结构域和抗原表位等信息。结果 SH3PXD2B基因编码长度为911aa的蛋白,具有1个PX和4个SH3结构域,蛋白理论分子量为101579.0,理论等电点为8.82。蛋白结构中α螺旋占21.62%,β转角占6.70%,无规则卷曲占58.95%。蛋白结构中无信号肽,但有4个跨膜结构,和8处抗原表面位点。蛋白互作网络显示,与其互作蛋白多与细胞伪足形成和细胞粘附有关。进化分析显示家猪、家兔等与人SH3PXD2B基因相似性更高。结论利用生物信息学方法预测SH3PXD2B蛋白的结构和功能,能为其在口腔颌面头颈部功能的研究提供信息基础。
Objective To study the sequence information and protein structure of SH3PXD2B by using a variety of bioinformatics methods and to provide basis for further verifying the function of this gene in oral and maxillofacial head and neck. Methods The SH3PXD2B sequence was obtained from Genbank. The gene structure analysis was used to predict the subcellular localization, secondary structure, tertiary structure, functional domain and epitope of the protein. Results The SH3PXD2B gene encoded a 911aa protein with one PX and four SH3 domains. The theoretical molecular weight of SH3PXD2B gene was 101579.0 and the theoretical isoelectric point was 8.82. The α-helix accounted for 21.62% of the protein structure, the β-angle accounted for 6.70%, and the irregular curl accounted for 58.95%. There are no signal peptides in the protein structure, but there are 4 transmembrane structures and 8 antigen surface sites. The protein interaction network shows that the interaction proteins with it are mostly related to cell pseudopodia formation and cell adhesion. Evolutionary analysis showed that domestic pigs, rabbits and other human SH3PXD2B gene similarity higher. Conclusion The prediction of the structure and function of SH3PXD2B protein using bioinformatics methods can provide an information basis for the study of the function of SH3PXD2B protein in oral and maxillofacial head and neck.