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目的探讨GB对体外培养的胚鼠脊髓神经元存活和生长发育的作用。方法胚胎大鼠脊髓神经细胞原代培养,相差显微镜下进行细胞计数和显微测量,观察GB对神经元存活和生长发育的作用。对培养7 d细胞行NSE免疫组化染色,光镜下观察GB对NSE染色阳性神经元生长发育的影响。结果GB及NGF能够促进体外培养胚胎大鼠脊髓神经细胞及NSE染色阳性神经元的存活,促进其细胞及突起的生长。其促进脊髓神经细胞的存活、胞体及突起发育的作用与NGF一致,而其促进神经突起分化与生长的作用强于NGF。结论NGF和GB能促进培养大鼠发育期脊髓神经元存活、分化和生长,并且表现出各自作用的特异性。
Objective To investigate the effect of GB on the survival and growth of cultured rat spinal cord neurons. Methods The primary culture of rat spinal cord neurons was performed. The cell counts and microscopic measurements were performed under a phase contrast microscope to observe the effect of GB on neuron survival and growth. NSE immunohistochemical staining was performed on cultured cells for 7 days. The effect of GB on the growth and development of NSE-positive neurons was observed under light microscope. Results GB and NGF could promote the survival of embryonic rat spinal cord neurons and NSE staining positive neurons in vitro, and promote the growth of their cells and processes. Its role in promoting the survival, cell body and process development of spinal cord neurons is consistent with NGF, and its role in promoting differentiation and growth of neurites is stronger than that of NGF. Conclusion NGF and GB can promote the survival, differentiation and growth of spinal cord neurons during the development of rats, and show their specific effects.