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鉴定了从浙江省各地采集的60株水稻纹枯病菌株的菌丝融合群归属情况。该60个菌株均与AG-1融合群的3个亚群AG-1-ⅠA、AG-1-ⅠB、AG-1-ⅠC的标准菌株融合,当属AG-1融合群。其中,有58株均与标准菌株AG-BⅠ融合,所有菌株与其他标准菌株均无融合现象。通过离体接种对菌株致病力进行鉴定,发现各菌株间致病力差异显著。致病力最强的TTZF-1与其中52个菌株差异极为显著。进一步的转录间隔区(ITS)序列分析显示,系统发育树的结果与融合群鉴定的结果一致,与菌株间致病力差异无相关性。
Sixty isolates of Rhizoctonia solani collected from all over Zhejiang Province were identified as belonging to the mycelial fusion group. The 60 isolates belonged to AG-1 fusion group which fused with the standard strains AG-1-ⅠA, AG-1-ⅠB and AG-1-ⅠC of three subgroups of AG-1 fusion group. Among them, 58 strains were all fused with the standard strain AG-BⅠ, and all the strains had no fusion with other standard strains. Pathogenicity was identified by in vitro inoculation and found that the pathogenicity of each strain was significantly different. The most virulent TTZF-1 and 52 strains of the difference is extremely significant. Further analysis of the ITS sequences showed that the phylogenetic tree was consistent with the identification of the fusarium and had no correlation with the pathogenicity of the isolates.