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The energy transfer and upconversion of Er3+/Yb3+ co-doped TeO2-TiO2-K2O glasses upon excitation with 976 nm lasers diode were studied. The tellurite glasses were prepared by conventional melting methods. Their optical properties and sensitization upconversion spectra were performed. The dependence of green upconversion luminescence intensity on the mole ratio of Yb3+ to Er3+ and Er3+ concentration were discussed in detail. When the mole ratio of Yb3+ to Er3+ is 25/1 and Er3+ concentration is 0.1% (mole fraction), or when the mole ratio of Yb3+ to Er3+ is 10/1 and Er3+ concentration is 0.15%, the optimal upconversion luminescence intensity is obtained. The obtained glasses can be one of the potential candidates for lasers-diode pumping microchip solid-state lasers.