本研究对６６例不明原因的原发不育及３４例继发不育患者的精液标本，同时进行去透明带地鼠卵穿透试验（ＨＯＰ）精子尾部低渗肿胀试验（ＨＯＳ）与ＤＮＡ荧光染色的有效精子计数（ＥＳＣ）的检测和统计分析。结果在１００例中ＨＯＳ与ＨＯＰ以及ＥＳＣ与ＨＯＰ的符合率分别达到７８％与７４％；ＨＯＳ与ＥＳＣ与ＨＯＰ三者的符合率亦达到６８％。表明ＨＯＳ或ＥＳＣ与ＨＯＰ一样也有反映精子受精能力的作用，并认为ＨＯＳ与ＥＳＣ的标准分别采用≥６０％与≥２０×１０６较为合适。此外，继发不育组的ＨＯＳ与ＨＯＰ符合率，ＥＳＣ与ＨＯＰ符合率以及ＨＯＳ与ＥＳＣ与ＨＯＰ三者符合率还显著高于原发不育组。 In this study, 66 cases of unexplained primary infertility and 34 cases of secondary infertility were also studied. At the same time, HOP and Hypothalamic fluorescence (HOS) Dyeing effective sperm count (ESC) detection and statistical analysis. Results The coincidence rates of HOS, HOP, ESC and HOP were 78% and 74% respectively in 100 cases. The coincidence rate of HOS, ESC and HOP was 68%. It shows that HOS or ESC, like HOP, also reflects the ability of spermatozoa to fertilize. It is considered that the standard of HOS and ESC should be ≥60% and ≥20 × 106, respectively. In addition, the coincidence rate of HOS and HOP, the coincidence rate of ESC and HOP and the coincidence rate of HOS and ESC and HOP in the secondary infertility group were also significantly higher than those in the primary infertility group.