免疫金银法是迄今免疫组化中最敏感的方法之一,它以其制备简单、不接触致癌物质等优点受到应用者的重视。但其显色需在暗室内进行,背景染色不易控制,使其应用带来不便。我们自制了一种“显色罐”,用于免疫金银染色法的显色,取得了良好的效果。 1 材料和方法 1.1 显色罐的制作 利用洗照片用的不锈 Immunogold-silver method is one of the most sensitive immunohistochemical methods so far, and has attracted the attention of users due to its simple preparation and non-contact with carcinogenic substances. However, its color needs to be carried out in a dark room, background dyeing is not easy to control, causing inconvenience to its application. We made a “color tank” for the colorimetric immunoluminescence staining, and achieved good results. 1 Materials and methods 1.1 The production of color tank Use wash photo with stainless steel