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目的研究大黄素致大鼠药物性肝损伤过程中机体肝功能情况。方法 Wistar雄性大鼠100只,随机分成5组,按照每天大黄素摄入量不同,分为高剂量组(16 mg/kg·d-1)、较高剂量组(8 mg/kg·d-1)、中剂量组(4 mg/kg·d-1)、低剂量组(2 mg/kg·d-1)、正常组(灌胃等体积蒸馏水),每组20只,连续灌胃12周,于4周、8周各组分别解剖5只,至12周解剖剩余全部大鼠,观察体质量及肝脾指数的变化;采用全自动生化检查肝功能;肝组织炎症情况采用肝组织HE染色进行观察。结果各组大鼠生长状态无明显差异,均无死亡和腹水形成。在体质量方面,较高剂量组和高剂量组有下降趋势,尤其是高剂量组体质量下降更显著;在肝脾指数方面,较高剂量组和高剂量组的肝脾指数均有增加趋势,尤其是高剂量组更显著。另外,较高剂量组和高剂量组的血清总胆红素(total bilirubin,TBil)、丙氨酸氨基转移酶(alanine aminotransferase,ACT)及天冬氨酸氨基转移酶(aspartate aminotransferase,AST)均有增加趋势,尤其是高剂量组增加更明显,至12周达到最大,而白蛋白水平各组差异不显著。对肝组织进行HE染色情况进行研究,发现各组在4周和8周时差异不明显,而在12周时较高剂量组和高剂量组肝组织炎症活动明显,尤其高剂量组更显著。结论采用大黄素口服可以诱导出大鼠肝损伤,有必要进一步研究。
Objective To study the liver function of emodin-induced liver injury in rats. Methods 100 male Wistar rats were randomly divided into 5 groups. According to the daily intake of emodin, they were divided into high dose group (16 mg / kg · d-1) and high dose group (8 mg / kg · d- 1), middle dose group (4 mg / kg · d-1), low dose group (2 mg / kg · d-1) and normal group (intragastric administration of equal volume distilled water) Week, at 4 weeks and 8 weeks, 5 rats were dissected in each group, and all the rats were dissected until 12 weeks to observe the change of body weight and index of liver and spleen. The liver function was examined by automatic biochemistry. Dyeing was observed. Results The growth status of rats in each group showed no significant difference, no death and ascites formation. In terms of body weight, the higher dose group and the high dose group showed a downward trend, especially in the high dose group, with a significant decrease in body mass; in the liver and spleen index, the liver and spleen index increased in both the high dose group and the high dose group , Especially high-dose group more significant. In addition, the levels of total bilirubin (TBil), alanine aminotransferase (ACT) and aspartate aminotransferase (AST) in the high and high dose groups Increasing trend, especially in the high-dose group increased more clearly, reaching the maximum at 12 weeks, while the albumin level in each group was not significantly different. Hematoxylin and eosin (HE) staining of liver tissue showed that there was no significant difference between the groups at 4 and 8 weeks, but at 12 weeks, liver tissue inflammatory activity was significantly higher in the high and high dose groups, especially in the high dose group. Conclusion Oral emodin can induce liver injury in rats, it is necessary to further study.