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本实验用低硒大鼠对人工栽培富硒平菇作了硒生物利用率观察。大鼠按体重随机分为9组,其中1组为低硒对照组,2~5组补充Na_2SeO_3,饲料内硒含量分别为:0.51、0.75、1.91、3.18ppm;6~9组补充富硒平菇,硒为0.45、0.72、1.59、3.41ppm。3周后处死大鼠,采血、肝、测定硒含量,并测定红细胞GSH-Px酶活性。结果表明:血、肝硒值及红细胞GSH-Px酶活性均随补硒浓度的增加而增加,以亚硒酸钠为基准,富硒平菇硒生物利用率,按血硒指标为66.66%;按肝硒指标为125%;按红细胞GSH-Px酶活性指标为123.42%,说明人工栽培富硒平菇可作为极好的食物硒来源。
Selenium bioavailability of Se-enriched Pleurotus ostreatus in low-selenium rats was observed in this experiment. The rats were randomly divided into 9 groups according to their body weight. Among them, 1 group was low-selenium control group, 2 to 5 groups were supplemented with Na 2 SeO 3, selenium contents in diet were 0.51, 0.75, 1.91 and 3.18 ppm respectively; Mushroom, selenium 0.45,0.72,1.59,3.41ppm. After 3 weeks, the rats were killed, blood and liver were collected, and the selenium content was measured. The activity of GSH-Px in erythrocytes was determined. The results showed that blood, hepatic selenium and erythrocyte GSH-Px activities increased with the increase of selenium concentration. Based on sodium selenite, selenium bioavailability of selenium-enriched Pleurotus ostreatus was 66.66% according to blood selenium index. According to liver and selenium index was 125%; according to erythrocyte GSH-Px enzyme activity index was 123.42%, indicating that artificial cultivation of Se-Pleurotus ostreatus can be used as an excellent source of food selenium.