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目的:探讨影响绞股蓝药用有效成分三萜皂苷含量的因素,为绞股蓝种植、制药提供科学建议。方法:采用超声波提取和分光光度法,分析比较不同品种、不同组织、不同产地绞股蓝间总三萜皂苷含量的差异。结果:七叶绞股蓝叶片三萜皂苷含量(6.78%)显著高于五叶绞股蓝(3.69%),绞股蓝叶片中三萜皂苷的平均含量(4.72%)显著高于茎的平均含量(1.85%),广西南部产五叶绞股蓝叶片三萜皂苷含量(4.1%)高于广西北部产五叶绞股蓝(3.6%)。结论:为获得含量更高的三萜皂苷,实现最大经济效益,建议药农种植七叶绞股蓝,药材收购时宜叶茎分开。
Objective: To explore the factors influencing the content of triterpene saponin in Gynostemma pentaphyllum, providing scientific advice for planting Gynostemma pentaphyllum. Methods: Ultrasonic extraction and spectrophotometry were used to analyze the differences of total triterpenoid saponins content among different species, tissues and areas of Gynostemma pentaphyllum. Results: The content of triterpenoid saponins in the leaves of Gynostemma pentaphyllum (6.78%) was significantly higher than that of Gynostemma pentaphyllum (3.69%). The average content of triterpene saponins in Gynura divaricata (4.72%) was significantly higher than that of stems (1.85%), The content of triterpenoid saponin in the leaves of five-leaf Gynostemma pentaphyllum in southern Guangxi was higher than that of the pentaphyllum pentaphyllum (3.6%) in northern Guangxi. Conclusion: In order to obtain higher triterpene saponin, to achieve the greatest economic benefits, it is recommended that farmers cultivate seven leaves Gynostemma, medicinal herbs should be separated leaves and stems.