论文部分内容阅读
为动态观察沙鼠肾原代细胞双价肾综合征出血热灭活疫苗免疫动物后的免疫反应 ,采用人用剂量的 1/ 2 0肌内注射免疫小鼠 ,局部麻醉下采集尾血 ,分离血清 ,用间接酶联免疫吸附试验 (ELISA)测定血清抗体水平 ;处死动物时分离腹股沟淋巴结和脾脏淋巴细胞进行分类计数。结果显示 :双价肾综合征出血热疫苗在初次免疫后第 2针血清抗体有较大提高 ,并维持在高水平。流式细胞分类计数 ,疫苗注射组腹股沟淋巴结CD+ 4 细胞的免疫荧光强度显著高于对照组 (P<0 0 5 ) ,但脾脏的B细胞荧光强度却显著低于对照组 (P<0 0 5 ) ,提示接种该疫苗后细胞免疫亢进。表明沙鼠肾原代细胞双价肾综合征出血热灭活疫苗随接种时间的推移 ,产生良好的免疫反应。这种反应在免疫 2针后能够维持较高水平。该疫苗的免疫机理可能是通过局部淋巴结对抗原呈递来实现的。同时提示 ,疫苗可能造成细胞免疫功能亢进
In order to dynamically observe the immune response of mice immunized with hemorrhagic fever with dengue virus immunodeficiency virus after primary degeneration of gerbil primary kidney cells, mice were immunized intramuscularly with 1/2 0 human dose and the tail blood was collected under local anesthesia. Serum levels were measured by indirect enzyme-linked immunosorbent assay (ELISA) serum antibody levels; when the animals were killed inguinal lymph nodes and spleen lymphocytes were counted. The results showed that the first two doses of serum antibody to hemorrhagic fever with double-price syndrome of renal syndrome were greatly increased and maintained at a high level. The immunofluorescence intensity of CD + 4 cells in inguinal lymph nodes in vaccinated group was significantly higher than that in control group (P <0.05), but the fluorescence intensity of B cells in spleen was significantly lower than that in control group (P <0.05 ), Suggesting that the vaccine immunization after cellular immune hyperfunction. Show that gerbil primary kidney cell degeneration double-degenerative renal syndrome vaccine with the passage of time, have a good immune response. This response can be maintained at a high level after 2 doses of immunization. The immune mechanism of the vaccine may be achieved by local lymph node antigen presentation. At the same time suggest that the vaccine may cause cellular immune function hyperthyroidism