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目的 :探讨红景天抗老龄大鼠嗅球衰老的机制。方法 :取健康大鼠 ,青龄组 (3个月 ) 10只 ;老龄组(2 6个月 ) 2 0只 (随机分成老龄对照组、老龄红景天组各 10只 ) ;均断头处死 ,取嗅球 ,经石蜡包埋 ,连续切片 ,免疫组化染色 ,光镜观察。结果 :成纤维细胞生长因子 (FGF)及凋亡相关基因 (Bcl 2和Bax)主要表达在大鼠嗅球的僧帽细胞 ;青龄组FGF和Bcl 2阳性细胞表达率均显著高于老龄对照组 (P <0 .0 1) ,且FGF和Bcl 2蛋白的表达呈明显正相关 (P <0 .0 1) ;而青龄组Bax阳性细胞表达率略低于老龄对照组 ,但差异不显著 (P >0 .0 5 )。老龄红景天组FGF和Bcl 2蛋白阳性细胞表达率明显高于老龄对照组 (P <0 .0 1)。结论 :大鼠嗅球老化与FGF和Bcl 2蛋白表达减少密切相关 ;推测红景天可能是通过上调嗅球中FGF蛋白的表达 ,诱导僧帽细胞Bcl 2蛋白表达增多 ,抑制嗅球中僧帽细胞的凋亡是其抗大鼠嗅球衰老的重要机制。
Objective: To explore the mechanism of aging of olfactory bulbs in Rhodiola rats. METHODS: Ten healthy rats (3 months) were used in the young age group and 20 were in the old age group (26 months) (randomly divided into old age control group and 10 old rhodiola group); The olfactory bulbs were taken and paraffin-embedded, serially sectioned, immunohistochemically stained, and light microscopy observed. RESULTS: Fibroblast growth factor (FGF) and apoptosis-related genes (Bcl 2 and Bax) were mainly expressed in rat olfactory bulb cap cells; the expression rates of FGF and Bcl 2 positive cells in young age groups were significantly higher than those in old controls. (P < 0.01), and the expression of FGF and Bcl 2 protein was significantly positively correlated (P <0. 01), while the expression rate of Bax positive cells in young age group was slightly lower than that of the old age control group, but the difference was not significant. (P > 0 .0 5 ). The expression of FGF and Bcl-2 protein positive cells in the aged Rhodiola group was significantly higher than that in the aged control group (P < 0.01). Conclusion: The aging of rat olfactory bulb is closely related to the decrease of FGF and Bcl 2 protein expression. It is speculated that Rhodiola may upregulate the expression of FGF protein in the olfactory bulb, induce the increase of Bcl 2 protein expression in mitochondrial cells, and inhibit the withering of the cap cells in the olfactory bulb. Death is an important mechanism for anti-rat olfactory bulb aging.